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Comparison of acid and enzymatic hydrolysis of pectin, as inexpensive source to cell growth of Cupriavidus necator Anais da ABC (AABC)
LOCATELLI,GABRIEL OLIVO; FINKLER,LEANDRO; FINKLER,CHRISTINE L.L..
Abstract: The present work investigated what the appropriate methods of hydrolysis of pectin for reducing compounds (RCs) production, employed as a substrate for cell growth of Cupriavidus necator. This microorganism has great importance industrial, because besides potential single cell protein (SCP), is the most studied microorganism for production of polyhydroxybutyrate (PHB), and both processes require high cell concentration with inexpensive substrates For this, it was compared to acid and enzymatic hydrolysis procedures, through rotational central composite experimental design, using pectin concentration (1.0%). It was analyzed as a variable response for both experimental design, the RCs’ production. The best conditions of each procedure were used...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cupriavidus necator; Galacturonic acid; Pectin depolymerization; Pectin hydrolysates; Polygalacturonase.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000300605
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Orange and Passion Fruit Wastes Characterization, Substrate Hydrolysis and Cell Growth of Cupriavidus necator, as Proposal to Converting of Residues in High Value Added Product Anais da ABC (AABC)
LOCATELLI,GABRIEL O.; FINKLER,LEANDRO; FINKLER,CHRISTINE L.L..
Abstract: Brazil is the world’s largest producer of orange and passion fruit, which are destined mainly for industrialization, generating grand volumes of wastes. The solid portion of these residues is a rich source of pectin - composed mainly of galacturonic acid and neutral sugars, which through the hydrolysis process can be used in biological conversion processes, as the production of polyhydroxyalkanoates (PHAs). This way, we characterized these wastes, followed by the extraction and hydrolysis of pectin for employ as a substrate for the cell growth of Cupriavidus necator. The results confirmed the large portion of pectin (almost 40 g.100g-1) and soluble sugars, present in these wastes. The hydrolyzed extract showed as a good source of carbon for the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological conversion processes; Enzymatic hydrolyses; Orange wastes; Passion fruit wastes; Pectin; Polyhydroxyalkanoates.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100629
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